1005 Low levels of IL-13 are constitutively produced in healthy skin and locally imprint a pro-type 2 immune response

To maintain an immunological balance and intact skin barrier a number of highly specialized immune cells shape anti-inflammatory pro-type 2 environment that can rapidly expel entrap multicellular pathogens, neutralize toxins support wound healing. Previous research has shown KLF4-expressing dendritic are drivers enhanced type immunity responsive the TSLP 1 interferon signaling. Single-cell profiling in healthy murine showed KLF4-expression was required by unique population dermal expressed low levels CD11c CD11b. Transcriptomic revealed this also displayed STAT6 associated gene signature not found other populations or tissues. In vivo, CD11b-low were absent from STAT6-KO mice, indicating localized activation. Analysis human cytokine production innate lymphoid cells, Dendritic Epidermal T Cells memory cells. skin, IL-13, but IL-4, necessary sufficient for development absence IL-13 signaling, stable continued to take up antigen, remained CD11b-hi. CD11b-hi furthermore defective activation response parasite antigens diminished ability IL-4+ GATA3+ Th2 cell differentiation. Low therefore play important role inducing reprogramming generation environment, which protects against pathogens might lead allergies.